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90
Oligos Etc for the nf-κb p65 shrna construct (5′-aggacctatgagaccttcaag-3′) and control shrna (empty vectors)
NF-κB activation regulates miR-574-5p expression in response to PM 2.5 exposure. ( a ) Binding of the <t>NF-κB</t> <t>p65</t> subunit to the promoter of the miR-574-5p gene. IgG was used as a negative control in the chromatin immunoprecipitation assay. ( b1 ) Knockdown of NF-κB p65 using a <t>shRNA</t> silencing technique eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( b2 ) Knockdown of NF-κB p65 by applying SC-514 (100 μM), an IKKβ specific inhibitor, eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c1 ) Knockdown of NF-κB p65 using a shRNA reverses the elevation in BACE1 in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c2 ) Knockdown of NF-κB p65 by applying SC-514 restores the BACE1 elevation in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. Green fluorescent protein (GFP) expression was used as a marker to indicate viral infection in the immunoblot assay. The data are expressed as the means ± SE ( n = 4 to 8). */ # P < 0.05; **/ ## P < 0.01; ***/ ### P < 0.001
For The Nf κb P65 Shrna Construct (5′ Aggacctatgagaccttcaag 3′) And Control Shrna (Empty Vectors), supplied by Oligos Etc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+shrna/for+the+nf+%CE%BAb+p65+shrna+construct++5++aggacctatgagaccttcaag+3+++and+control+shrna++empty+vectors+/pmc05575838-242-9-0
Average 90 stars, based on 1 article reviews
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90
Shanghai GenePharma control empty shrna
NF-κB activation regulates miR-574-5p expression in response to PM 2.5 exposure. ( a ) Binding of the <t>NF-κB</t> <t>p65</t> subunit to the promoter of the miR-574-5p gene. IgG was used as a negative control in the chromatin immunoprecipitation assay. ( b1 ) Knockdown of NF-κB p65 using a <t>shRNA</t> silencing technique eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( b2 ) Knockdown of NF-κB p65 by applying SC-514 (100 μM), an IKKβ specific inhibitor, eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c1 ) Knockdown of NF-κB p65 using a shRNA reverses the elevation in BACE1 in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c2 ) Knockdown of NF-κB p65 by applying SC-514 restores the BACE1 elevation in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. Green fluorescent protein (GFP) expression was used as a marker to indicate viral infection in the immunoblot assay. The data are expressed as the means ± SE ( n = 4 to 8). */ # P < 0.05; **/ ## P < 0.01; ***/ ### P < 0.001
Control Empty Shrna, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/control+empty+shrna/control+empty+shrna/pm34626772-105-8-14
Average 90 stars, based on 1 article reviews
control empty shrna - by Bioz Stars, 2026-11
90/100 stars
  Buy from Supplier

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NF-κB activation regulates miR-574-5p expression in response to PM 2.5 exposure. ( a ) Binding of the NF-κB p65 subunit to the promoter of the miR-574-5p gene. IgG was used as a negative control in the chromatin immunoprecipitation assay. ( b1 ) Knockdown of NF-κB p65 using a shRNA silencing technique eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( b2 ) Knockdown of NF-κB p65 by applying SC-514 (100 μM), an IKKβ specific inhibitor, eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c1 ) Knockdown of NF-κB p65 using a shRNA reverses the elevation in BACE1 in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c2 ) Knockdown of NF-κB p65 by applying SC-514 restores the BACE1 elevation in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. Green fluorescent protein (GFP) expression was used as a marker to indicate viral infection in the immunoblot assay. The data are expressed as the means ± SE ( n = 4 to 8). */ # P < 0.05; **/ ## P < 0.01; ***/ ### P < 0.001

Journal: Particle and Fibre Toxicology

Article Title: NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM 2.5 aspiration

doi: 10.1186/s12989-017-0215-3

Figure Lengend Snippet: NF-κB activation regulates miR-574-5p expression in response to PM 2.5 exposure. ( a ) Binding of the NF-κB p65 subunit to the promoter of the miR-574-5p gene. IgG was used as a negative control in the chromatin immunoprecipitation assay. ( b1 ) Knockdown of NF-κB p65 using a shRNA silencing technique eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( b2 ) Knockdown of NF-κB p65 by applying SC-514 (100 μM), an IKKβ specific inhibitor, eliminates the reduction in miR-574-5p following 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c1 ) Knockdown of NF-κB p65 using a shRNA reverses the elevation in BACE1 in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. ( c2 ) Knockdown of NF-κB p65 by applying SC-514 restores the BACE1 elevation in response to 10 μg/mL PM 2.5 treatment in primary cultured hippocampal neurons. Green fluorescent protein (GFP) expression was used as a marker to indicate viral infection in the immunoblot assay. The data are expressed as the means ± SE ( n = 4 to 8). */ # P < 0.05; **/ ## P < 0.01; ***/ ### P < 0.001

Article Snippet: Oligos for the NF-κB p65 shRNA construct (5′-AGGACCTATGAGACCTTCAAG-3′) and control shRNA (empty vectors) were used for knockdown of NF-κB p65.

Techniques: Activation Assay, Expressing, Binding Assay, Negative Control, Chromatin Immunoprecipitation, Knockdown, shRNA, Cell Culture, Marker, Infection, Western Blot